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L0881
Sigma
Lectin from Arachis hypogaea (peanut)
lyophilized powder, Affinity-purified
| Synonym: | Peanut agglutinin, PNA |
Description
| Analysis Note | Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of neuraminidase treated human blood group O erythrocytes after 1 hr incubation at 25 °C. |
| Biochem/physiol Actions | PNA does not agglutinate normal human erythrocytes, but strongly agglutinates neuraminidase treated erythrocytes. PNA has potent anti-T activity similar to the anti-T antibody in human sera. The lectin can be used to distinguish between human lymphocyte subsets. |
Properties
| form | lyophilized powder |
| potency | <0.1 μg per mL agglutination activity |
| quality | Affinity-purified |
| total impurities | salt, free |
| storage temp. | −20°C |
Safety
| Personal Protective Equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
| WGK Germany | 3 |
References
| reference | Lotan, R. J. Biol. Chem. 250, 8518, (1975) |
| London, J. J. Immunol. 121, 438, (1978) | |
| Bird, G. W. G. Vox Sang. 9, 748, (1964) |
